Minister of Applecross. Son of the Donald Macqueen, Minister of Kilmuir. Married (1781) to Jane Macrae, daughter of Farquhar Macrae of Inverinate, with whom he had several children.
John Macqueen was educated at King's College, Aberdeen. He was ordained Minister of Applecross, a peninsula on the Scottish mainland opposite Skye and Raasay, on August 13, 1777.
Boswell and Dr Johnson met Macqueen at Raasay on September 8, 1773. They had been accompanied to the island by his father, Donald Macqueen, earlier that same day.